•
luciferase
英：【lu:sɪfəreɪs】美：【lu:sɪfəˌreɪs】
英：
美：
n.
【生】萤光素酶
1 、A chemical substance present in the cells of bioluminescent organisms, such as fireflies, that produces an almost heatless, bluish - green light when oxidized under the catalytic effects of
luciferase
.───荧光素存在于生物性发光有机体（比如萤火虫）中的一种化学物质，它在荧光素酶催化作用下氧化时发生几乎没有热量的蓝绿光
2 、DTA vector containing the binding sequence of EWS-FLI1 depresses the activation of
luciferase
───含EWS-FLI1结合序列的DTA载体对报告基因表达抑制的研究
3 、The fact that PTC124 binds to
luciferase
, he says, "has nothing to do with whether its a good drug.───他认为，事实上PTC124连接上荧光素酶后，“并任何迹象显示出它是一种好药。”
4 、The
luciferase
activity of lysate expressing FLAG-pcDNA3-N was lower than that expressing FLAG-pcDNA3.Addition of N protein in RRL system decreased
luciferase
activity.───构建了载体FLAG-pcDNA3-N,在293T人胚肾细胞内表达后,荧光素酶活性被抑制; 在体外翻译体系中加入N蛋白,体外翻译被抑制。
5 、Approximately, 1.5- and 2.5-fold elevated levels of
luciferase
activity relative to untreated cells were detected in E. coli/pLux2 (UAG) and pLux3 (UAA) when gentamicin was present at 0.5 Ag/ml.───与未处理细胞相比，0.5Ag/ml浓度的庆大霉素能够使pLux2(UAG)大肠杆菌和pLux3(UAA)大肠杆菌的荧光素酶活性提高1.5-2.5倍。
6 、All three mutants showed higher
luciferase
activity than that of the wild type, and the V85I mutant showed the highest activity.───三个突变异种均显示比野生型具有更高的荧光素酶活性，并且V85I突变异种活性最强。
7 、96-Well Plate Dual Luciferase Assay───96孔板荧光素酶检测
8 、Keywords Ewing family of tumor;fusion gene;diphtheria toxin A fragment （DTA）;
luciferase
;───尤文家族肿瘤;融合基因;白喉毒素A链;虫荧光素酶;
9 、Dr. Geri Dai, Promega Corporation Luciferase Gene Reporter System and Other Applications───利用萤光素酶做报告基因和其它分子生物学检测
10 、Here, it was found that actin and
luciferase
nascent chains were adjacent to more than one TRiC subunit at different stages of translation.───尽管多方面表示混沌理论具有明显的可应用性于现代企业问题与供应链管理领域，然而，在这方面的应用仍是有限。
11 、In the natural world, a chemical called luciferin is mixed with an enzyme called
luciferase
.─── 在自然界中 一种叫荧光素的化学物质 和荧光素酶混合
12 、Keywords lentivirus vector;
luciferase
;bioluminescence imaging;───慢病毒载体;荧光素酶;生物素荧光成像;
13 、Eriogyna pyertorum lucifera───n. 樟蚕四川亚种
14 、Keywords : Hepatitis C virus;Antisense oligodeoxynucleotide;Transgene;Luciferase;───肝炎病毒.丙型;寡核苷酸.反义;转基因;荧光素酶;
15 、Expression of
luciferase
gene of pcDNA3 OCP Luc is specific to rhBMP 2 and can be used for the quantitative bioassay of rhBMP 2.───真核表达载体 pc DNA3- OCP- L uc的报告基因表达具有特异性 ,可代替直接测定骨钙素用于 rh BMP- 2活性的定量测定研究 .
16 、Keywords glioma;mesenchymal stem cells （MSCs）;lentivirus;bioluminescence imaging （BLI）;
luciferase
;tropism;───胶质瘤;骨髓间充质干细胞;慢病毒;生物发光成像;荧光素酶;迁移;
17 、Establishment of cell model expressing HCV-C and
luciferase
fusion protein by microinjection───显微注射法建立含荧光素酶和HCV-C基因细胞模型
18 、Most compounds identified by the firefly
luciferase
screen do not interact in this way with
luciferase
, he says, but it is important to make sure.───他认为，绝大多数的化合物能被荧光素酶识别，但不会发生和荧光素酶发生这种相互作用，但这必须得到确认。
19 、Keywords bioluminescent imaging;
luciferase
;polyethylenimine;promoter;suicide gene;ovarian cancer;───生物荧光成像;荧光素酶;聚乙烯基亚胺;启动子;自杀基因;卵巢癌;
20 、
luciferase
reporter gene activity───报告基因活性
21 、The constructed wild type p21 gene promoter driven
luciferase
repo rter plasmid is used to transfect ECV304 cells to express
luciferase
protein sta bly and reliably.───构建的野生型p21基因启动子驱动的荧光素酶报告基因载体,转染ECV304细胞后能够表达荧光素酶蛋白,具有稳定和可靠性能。
22 、Keywords proline-rich nuclear receptor coactivator（PNRC）;
luciferase
assays;promoter characterization;───富含脯氨酸核受体辅活化子（PNRC）;荧光素酶活性检测;启动子鉴定;
23 、18. The transcriptional activation activity of GR was evaluated through method of relative activity of
luciferase
.───相对荧光素酶活性法检测GR转录激活功能的变化。
24 、I think weve mentioned them already, luciferin and
luciferase
.─── 就是之前已经提到过的 荧光素和荧光素酶
25 、Laboratories can employ
luciferase
to emit light in the presence of certain diseases.───医院用荧光素酶的发光来发现特定的疾病。
26 、Which, when catalyzed by
luciferase
, causes the bones to glow.─── 会被荧光素酶催化 让骨头发光
27 、Sites that are surrounded by parentheses are not unique, as additional sites for each also exists in the
luciferase
gene.───括号中的限制性内切酶位点不是唯一的，因为他门在荧光素酶基因上也存在。
28 、Constructing and Identifying the Firefly Luciferase Report Gene Vectors For 5Flanking Regulated Elements of NGAL Gene───NGAL基因5侧翼区转录调控元件萤火虫荧光素酶报告基因表达载体的构建与鉴定
29 、Banaiee N.Bobadilla del VM.Bardarov S Luciferase reporter mycobacteriophages for detection,identification,and antibiotic susceptibility testing of Mycobacterium tuberculosis in Mexico 2001───吕斌.徐顺清.符志军.陈志飞.李开赏.周宜开重组分枝杆菌噬菌体检测分枝杆菌的发光研究【期刊论文】-微生物学免疫学进展2001(1
30 、Keywords NGAL gene;regulation of gene expression;
luciferase
;report gene;construction vector;───关键词NGAL基因;基因表达调控;荧光素酶;报告基因;载体构建;
31 、Keywords RNA interference;
luciferase
;osteosarcoma;───RNA干扰;荧光素酶;骨肉瘤;
32 、prokaryotic
luciferase
───原核生物虫萤光素酶
33 、As leucine zipper is a basic DNA binding motif of many known transcription factors, we examined the transcriptional function of the TCP10L using dual
luciferase
assay system.───本研究的结果进一步加深了对人体MT基因结构与功能的认识，也为阐明HCC发生的分子机制提供了有重要价值的资料。
34 、When paromomycin was used at 2.5 Ag/ml,
luciferase
activity was elevated 8- and 10-fold in E. coli/pLux2 (UAG) and pLux3 (UAA) relative to untreated cells.───当巴龙霉素的浓度达到2.5Ag/ml时，相对于未处理的对照组细胞，大肠杆菌/pLux2(UAG)和大肠杆菌/pLux3(UAA)的荧光素酶活性增加了8-10倍。
35 、Thus we constructed reporter vectors in which the reporter gene is
luciferase
.───经酶切鉴定以及测序分析确定载体构建正确。
36 、Methods The human brain tumor cell line U87MG cells were transduced by using the SMPU-R-MND-luc vector then a clone with high
luciferase
activity was cloned and used for in vivo experiments.───方法用SMPU-R-MND-luc载体转染人脑肿瘤U87MG细胞系,形成具有高荧光素酶活性的细胞克隆。
37 、Keywords gene transfer;electric pulse;
luciferase
;───基因转移;电脉冲;荧光素酶;
38 、Keywords Dunaliella salina;
luciferase
gene;expression plasmid vectors;───杜氏盐藻;荧光素酶基因;质粒表达载体;
39 、Keywords dengue virus;replicon;Renilla
luciferase
;───登革病毒;复制子;海肾萤光素酶;
40 、fast
luciferase
automated assay of specimens for hospitals───医院标本快速荧光素酶自动测定
41 、The researchers developed a new screening system, based on a naturally fluorescent protein called
luciferase
, to identify small molecules that inhibit proteins that turn genes on and off.───在天然荧光蛋白荧光素酶的基础上，研究者发展了一种新的筛选系统，此系统可以用来鉴定抑制启动和关闭基因蛋白的小分子。
42 、Addition of N protein in RRL system decreased
luciferase
activity.───在体外翻译体系中加入N蛋白,体外翻译被抑制。
43 、Keywords hepatitis C virus;cellular model;
luciferase
;ribozyme;───丙型肝炎病毒;细胞模型;荧光素酶;核酶;
44 、Light is emitted when
luciferase
is exposed to the appropriate luciferin substrate.Photon emission can be detected by light sensitive apparatus such as a luminometer or modified optical microscopes.───在荧光素酶中加入正确的荧光素底物就可以放出荧光，而发出的光子可以被光敏感元件，如荧光探测器或改进后的光学显微镜探测到。
45 、cypridina
luciferase
───海萤萤光酶
46 、Luciferase can be used in blood banks to determine if red blood cells are starting to break down.───例如，荧光素酶可以被用于检测血库中所存血液中的红血球是否开始破裂。
47 、Luciferase expression in E. coli/pLux1 was not affected by paromomycin at sub-growth inhibitory concentrations.───反之，在低于生长抑制的浓度下，巴龙霉素不影响大肠杆菌pLux1基因荧光素酶的表达。
48 、Keywords bioluminescence;total bacterial count;ATP;luciferin;
luciferase
;───生物发光;细菌总数;三**酸腺苷;荧光素酶;荧光素;
49 、Comparison of the relative
luciferase
activity in secondary CEF by different heterogenous strong promoters, MDV gB promoter and the composed promoters───启动子及其复合启动子的活性比较
50 、Keywords translation initiation region;
luciferase
;secondary structure;expression;───翻译起始区;荧光素酶;二级结构;表达;
51 、Keywords PDGF-D;Single nucleotide polymorphism;Stroke;Haplotype;Promoter;Luciferase Report Gene System;───单核苷酸多态性;脑卒中;单体型;血小板源性生长因子D;启动子;荧光素酶报告系统;
52 、Cotransfection of GalT I promoter /
luciferase
reporter and Ets-1 expression plasmid increased the
luciferase
reporter activity in a dose dependent manner.───我们采用荧光素酶报告基因的方法证实Ets-1可激活 GalT I报告基因的表达，并具有量效关系。
53 、30. The transcriptional activation function of GR was evaluated through the detection of relative
luciferase
activity after dexamethasone treatment.───相对荧光素酶活性法检测地塞米松作用后GR转录激活功能的变化。
54 、ATP occurs wherever bacteria are present.Reaction of ATP with Luciferin Firetly in the presence of Luciferase results in the emission of light as a glow or flash.───ATP化验ATP和虫荧光素就是萤火虫当闪动的时候有细菌存在就有ATP存在。
55 、Inhibition of Luciferase expression in mammalian cells by AAV vector plasmid mediated Luciferase shRNA───AAV载体质粒介导的荧光素酶shRNA抑制其在哺乳动物细胞中的表达
56 、Keywords human telomerase reverse transcriptase（hTERT）;promoter;
luciferase
gene expression vector;───端粒酶催化亚基;启动子;荧光素酶基因表达载体;
57 、A chemical substance present in the cells of bioluminescent organisms,such as fireflies,that produces an almost heatless,bluish-green light when oxidized under the catalytic effects of
luciferase
.───荧光素存在于生物性发光有机体（比如萤火虫）中的一种化学物质，它在荧光素酶催化作用下氧化时发生几乎没有热量的蓝绿光。
58 、Keywords Environmental pollutants;Electrophile chemical pollutants;Firefly
luciferase
;Transgenic cell;───环境污染物;亲电性化学污染物;虫荧光素酶;转基因细胞;
59 、bacterial
luciferase
───细菌荧光素酶, 细菌虫荧光素酶
60 、Renilla-type
luciferase
───花虫型荧光素酶
61 、However, as in the case of gentamicin, no increase in
luciferase
activity was observed in E. coli/pLux4 (UGA).───但与庆大霉素的研究结果相同，大肠杆菌/pLux4(UGA)的荧光素酶活性没有增加。
62 、PCR. The MDK promoter/enhancer (MDKp) sequence with runt domain binding sites was amplified from the human genome DNA and directionally cloned into pGL3-Basic Luciferase reporter vectors to construct the MDKp-pGL3-B expression plasmids.───从基因组DNA中PCR扩增一段含Runt结合顺序的MDK基因启动子/增强子序列(MDKp)，定向克隆到pGL3-Basic质粒，构建MDKp-pGL3-Basic Luciferase报告基因表达载体；
63 、Inhibition of
luciferase
expression in hepatocellular carcinoma cells by short hairpin RNA───短发夹RNA抑制外源荧光素酶在肝癌细胞中的表达
64 、Gentamicin and paromomycin increased
luciferase
activity up to 2.5- and 10-fold, respectively.───抑制的作用机制很可能和通读的增强有关，但此假设还需要进一步验证。
65 、Keywords Drosophila S2 cells;
luciferase
;promoter;reporter gene;transfection;───果蝇S2细胞;荧光素酶;启动子;报告基因;转染;
66 、Then it was inserted into pBI-L vector to construct HGF and Smad7 expression vector containing
luciferase
reporter gene and respond element of tetracycline (TRE) by recombinant DNA technology.───利用DNA基因重组技术，将目的片段DNA**入含有荧光素酶报告基因和四环素反应元件的真核表达载体pBI-L中，分别构建真核表达载体pBI-L-HGF和pBI-L-Smad7，并进行酶切和测序鉴定。
67 、Keywords low density lipoprotein receptor;
luciferase
reporter gene;Traditional Chinese Medicine;drug screening;───低密度脂蛋白受体;荧光素酶报告基因;中药;药物筛选;
68 、Cypridina-type
luciferase
───海萤火虫型荧光素酶
69 、Keywords PLAGL2;SP-C promoter;
luciferase
activity;measurement;EMSA;───PLAGL2蛋白;SP-C启动子;荧光素酶报告基因检测;凝胶电泳迁移实验;
70 、The details of cloning, expression and function of biotinylated
luciferase
will be discussed in this paper.───本文将就生物素化荧光素酶的克隆、表达以及功能检测进行具体讨论。
71 、Since the mutant lossing the first 6 amino acids from the N terminus of firefly
luciferase
keeps 77% of natural
luciferase
activity, the 7th N terminal amino acid is of great importance to the normal enzymatic function.───由于N末端缺失6个氨基酸的突变体保持了77%的酶活性，因而萤火虫荧光素酶N末端第7个氨基酸与酶的催化活性密切相关。
72 、An alternative method uses bioluminescent proteins, such as the firefly enzyme
luciferase
, to detect pyrophosphate released when a base attaches to the primer strand.───另一种方法则利用了生物发光蛋白质，像是萤火虫的萤光素酶（luciferase），来侦测核苷酸连接在引子序列后所释放的焦**酸。
73 、9. 2. EWS-FLI-1 can regulate the expression of
luciferase
because of its powerful transcription activity.───尤文肉瘤特异性的EWS-FLI-1融合蛋白有强烈的转录活性，可以促进虫荧光素酶基因高表达。收藏指正
74 、Keywords
luciferase
;bioluminescence;RLU;disinfection effect;rapid evaluation;───关键词荧光素酶;生物发光法;RLU;消毒效果;快速评价;
75 、Construction of expression plasmid vectors containing the
luciferase
gene of an algae Dunaliella salina───杜氏盐藻荧光素酶基因表达载体的构建
76 、Keywords recombinant adeno-associated virus 2;firefly
luciferase
gene;transduction;gene therapy;───2型重组腺相关病毒;萤火虫荧光素酶基因;转导;基因治疗;
77 、The in vivo binding and functional relation between BRCA2 and FHL2 was demonstrated by coimmunoprecipitation, mammalian twohybrid assay and
luciferase
detection.───免疫共沉淀和哺乳动物细胞双杂交试验显示BRCA2和FHL2在体内特异性结合，并证实FHL2在体内形成同源二聚体；
78 、Construction of a
luciferase
report vector with prolatin regulating sequence───催乳素调控序列荧光素酶报告基因的构建
79 、EWS-FLI-1 can regulate the expression of
luciferase
because of its powerful transcription activity.───尤文肉瘤特异性的EWS-FLI-1融合蛋白有强烈的转录活性，可以促进虫荧光素酶基因高表达。
80 、Renilla
luciferase
───海肾萤光酶
81 、Effects of Translation Initiation Region Secondary Structure on Expression of Luciferase Gene in COS-7 Cells───翻译起始区二级结构对荧光素酶基因在COS-7细胞中表达效率的影响
82 、By measuring the quantities of
luciferase
and green fluorescence protein,we can validate whether the models are right or effective.───据此，我们初步认为，水溶成分中存在着该种中药的活性成分。
83 、Plasmids of the pCMV5-AML1/AML1-ETO and the MDKp-pGL3-B or mu-MDKp-pGL3-B were then co-transduced into 293T cells and the
luciferase
relative value was analyzed.───将正常和突变型表达载体与AML1或AML1-ETO基因瞬时共转染293T细胞，分析/LML1、AML1-ETO对报告基因的转录调节作用。
荧光素酶发光原理？
利用荧光素酶与底物结合发生化学发光反,应的特点，把感兴趣的基因转录的调控元件克隆在萤火虫荧光素酶基因(fireflyluciferase)的. 上游，构建成荧光素酶报告质粒。
然后转染细胞，适当刺激或处理后裂解细胞,测定荧光素酶活性。通过荧光素酶活性的高低判断性刺前后或不同刺激对感兴趣的调控元件的影响。
同时，为了减少内在的变化因素对实验准确性的影响，将带有海肾荧光素酶基因(Rinilla luciferase)的质粒作为对照质粒与报告基因质粒共转染细胞，提供转录活力的内对照，使测试结果不受实验条件变化的干扰。
荧光素酶发光原理？
萤火虫荧光素酶在氧气、ATP和镁离子同时存在的条件下，催化荧光素氧化，生成氧化荧光素，同时产生黄绿色光，波长540-600nm。
荧光素酶（luciferase）是自然界中能够产生生物荧光的酶的总称。荧光素酶可以催化荧光素氧化成氧化荧光素，在荧光素氧化的过程中，会发出生物荧光。然后可以通过荧光测定仪测定荧光素氧化过程中释放的生物荧光。荧光素和荧光素酶这一生物发光体系，可以极其灵敏、高效地检测基因的表达，是检测转录因子与目的基因启动子区DNA相互作用的一种检测方法。
目前，最有代表性的是从甲虫中分离得到的萤火虫荧光素酶（Firefly luciferase）和从海肾中分离得到的海肾荧光素酶（Renilla luciferase）。